PrimePCR™ Probe Assay: CUTA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CUTA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CUTA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040710
Assay Design:   exonic
Chromosome Location:   6:33385273-33385412question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000487148 ENST00000440930 ENST00000435267 ENST00000561208 ENST00000338564 ENST00000397624 ENST00000559085 ENST00000397620 ENST00000424352 ENST00000559561 ENST00000382766 ENST00000444725 ENST00000340545 ENST00000219869 ENST00000374500 ENST00000488034 ENST00000494751 ENST00000488478 ENST00000374496 ENST00000440279

Gene Information

Description Not Available

Gene Symbol:   CUTA
Gene Name:   cutA divalent cation tolerance homolog (E. coli)
Aliases:   ACHAP, C6orf82, MGC111154
RefSeq:   NC_000006.11 NG_016137.1 NT_007592.15 NT_167249.1
Ensembl:   ENSG00000112514
Entrez:   51596
Chromosome Mapping:   6p21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.860000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download