This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: NDUFS8, Human
PrimePCR™ Template for Probe Assay: NDUFS8, Human
This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase or Complex I a multimeric enzyme of the respiratory chain responsible for NADH oxidation ubiquinone reduction and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and as such is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. [provided by RefSeq Mar 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.