PrimePCR™ Probe Assay: IFT20, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040703
Assay Design:   exonic
Chromosome Location:   17:26658895-26659002question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000583970 ENST00000583880 ENST00000581127 ENST00000580696 ENST00000577872 ENST00000575742 ENST00000573233 ENST00000581681 ENST00000580505 ENST00000579419 ENST00000578009 ENST00000357896 ENST00000395418 ENST00000585089 ENST00000585313 ENST00000578122 ENST00000578985 ENST00000577498

Gene Information

Description Not Available

Gene Symbol:   IFT20
Gene Name:   intraflagellar transport 20 homolog (Chlamydomonas)
Aliases:   Not Available
RefSeq:   NC_000017.10 NT_010799.15
Ensembl:   ENSG00000109083
Entrez:   90410
UniGene:   Hs.705431
Chromosome Mapping:   17q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.530000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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