PrimePCR™ Probe Assay: BCL7B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BCL7B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BCL7B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040698
Assay Design:   exonic
Chromosome Location:   7:72951347-72951494question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000357985 ENST00000263593 ENST00000545756 ENST00000378295 ENST00000378288 ENST00000377813 ENST00000360807 ENST00000377807 ENST00000246043 ENST00000455029 ENST00000379264 ENST00000379246 ENST00000379240 ENST00000431707 ENST00000379272 ENST00000544770 ENST00000589334 ENST00000206380 ENST00000409671 ENST00000389176 ENST00000595464 ENST00000600626 ENST00000257267 ENST00000477854 ENST00000358540 ENST00000396170 ENST00000396168 ENST00000396171 ENST00000396167 ENST00000304166 ENST00000326654 ENST

Gene Information

This gene encodes a member of the BCL7 family including BCL7A BCL7B and BCL7C proteins. This member is BCL7B which contains a region that is highly similar to the N-terminal segment of BCL7A or BCL7C proteins. The BCL7A protein is encoded by the gene known to be directly involved in a three-way gene translocation in a Burkitt lymphoma cell line. This gene is located at a chromosomal region commonly deleted in Williams syndrome. This gene is highly conserved from C. elegans to human. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Oct 2010]

Gene Symbol:   BCL7B
Gene Name:   B-cell CLL/lymphoma 7B
Aliases:   Not Available
RefSeq:   NC_000007.13 NG_027959.1 NT_007933.15
Ensembl:   ENSG00000106635
Entrez:   9275
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.050000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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