PrimePCR™ Probe Assay: CCL24, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CCL24, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CCL24, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040695
Assay Design:   exonic
Chromosome Location:   7:75441173-75442633question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000572880 ENST00000319080 ENST00000538698 ENST00000352645 ENST00000351589 ENST00000215980 ENST00000404067 ENST00000402338 ENST00000407253 ENST00000356331 ENST00000442312 ENST00000503393 ENST00000373313 ENST00000376454 ENST00000307544 ENST00000396445 ENST00000376451 ENST00000458595 ENST00000376452 ENST00000341928 ENST00000303407 ENST00000371834 ENST00000357885 ENST00000410018 ENST00000409235 ENST00000409410 ENST00000215069 ENST00000523079 ENST00000325083 ENST00000517730 ENST00000519253 ENST

Gene Information

This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes a minimal activity on neutrophils and is negative on monocytes and activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. [provided by RefSeq Jul 2008]

Gene Symbol:   CCL24
Gene Name:   chemokine (C-C motif) ligand 24
Aliases:   Ckb-6, MPIF-2, MPIF2, SCYA24
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000106178
Entrez:   6369
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.750000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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