PrimePCR™ Probe Assay: BCAT2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BCAT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BCAT2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040689
Assay Design:   exonic
Chromosome Location:   19:49300448-49300545question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000537152 ENST00000425898 ENST00000327787 ENST00000304874 ENST00000380839 ENST00000395332 ENST00000395331 ENST00000402551 ENST00000316273 ENST00000593515 ENST00000597011 ENST00000599246 ENST00000598162 ENST00000545387

Gene Information

This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine isoleucine and valine. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2009]

Gene Symbol:   BCAT2
Gene Name:   branched chain amino-acid transaminase 2, mitochondrial
Aliases:   BCAM, BCATM, BCT2, PP18
RefSeq:   NC_000019.9 NT_011109.16 NG_013003.1
Ensembl:   ENSG00000105552
Entrez:   587
Chromosome Mapping:   19q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.680000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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