PrimePCR™ Probe Assay: HDAC10, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040680
Assay Design:   exonic
Chromosome Location:   22:50688856-50688948question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000394078 ENST00000508305 ENST00000239440 ENST00000504448 ENST00000220496 ENST00000424267 ENST00000216271 ENST00000448072 ENST00000349505 ENST00000400237 ENST00000400236 ENST00000260283 ENST00000393452 ENST00000554180 ENST00000393454 ENST00000553617 ENST00000335725 ENST00000555880 ENST00000357382 ENST00000298622 ENST00000375482 ENST00000416701 ENST00000337352 ENST00000287078 ENST00000335494 ENST00000361170 ENST00000392056 ENST00000344657 ENST00000400767

Gene Information

The protein encoded by this gene belongs to the histone deacetylase family members of which deacetylate lysine residues on the N-terminal part of the core histones. Histone deacetylation modulates chromatin structure and plays an important role in transcriptional regulation cell cycle progression and developmental events. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   HDAC10
Gene Name:   histone deacetylase 10
Aliases:   DKFZp761B039, HD10, MGC149722
RefSeq:   NC_000022.10 NT_011526.7
Ensembl:   ENSG00000100429
Entrez:   83933
UniGene:   Hs.26593
Chromosome Mapping:   22q13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.660000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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