PrimePCR™ Probe Assay: DDT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DDT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DDT, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040675
Assay Design:   exonic
Chromosome Location:   22:24315961-24316083question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000346085 ENST00000350026 ENST00000215770 ENST00000398344 ENST00000350608 ENST00000404092 ENST00000403754 ENST00000430101 ENST00000276440 ENST00000374667 ENST00000369682 ENST00000593808

Gene Information

D-dopachrome tautomerase converts D-dopachrome into 56-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence enzyme activity and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq Jul 2008]

Gene Symbol:   DDT
Gene Name:   D-dopachrome tautomerase
Aliases:   DDCT
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000099977
Entrez:   1652
Chromosome Mapping:   22q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.740000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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