PrimePCR™ Probe Assay: FAM21A, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040674
Assay Design:   exonic
Chromosome Location:   10:51863808-51866110question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000374362 ENST00000336378 ENST00000540872 ENST00000537517 ENST00000359860 ENST00000282633 ENST00000351071 ENST00000314664 ENST00000399339 ENST00000358474 ENST00000355876 ENST00000579270 ENST00000584292 ENST00000583993 ENST00000578828 ENST00000543972

Gene Information

Description Not Available

Gene Symbol:   FAM21A
Gene Name:   family with sequence similarity 21, member A
Aliases:   bA56A21.1
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000099290
Entrez:   387680
UniGene:   Hs.645272
Chromosome Mapping:   10q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.530000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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