PrimePCR™ Probe Assay: MT3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: MT3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MT3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040667
Assay Design:   exonic
Chromosome Location:   16:56624776-56624944question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000389811 ENST00000314666 ENST00000546020 ENST00000313735 ENST00000491289 ENST00000242208 ENST00000442711 ENST00000228567 ENST00000535526 ENST00000296873 ENST00000448933 ENST00000466444 ENST00000373233 ENST00000309279 ENST00000371929 ENST00000371916 ENST00000397452 ENST00000397450 ENST00000534972 ENST00000177694 ENST00000382592 ENST00000542899 ENST00000541194 ENST00000537928 ENST00000279593 ENST00000561640 ENST00000565838 ENST00000200691 ENST00000563979

Gene Information

Description Not Available

Gene Symbol:   MT3
Gene Name:   metallothionein 3
Aliases:   GIF, GIFB, GRIF
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000087250
Entrez:   4504
Chromosome Mapping:   16q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.910000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download