PrimePCR™ Probe Assay: SLC46A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLC46A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC46A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040661
Assay Design:   exonic
Chromosome Location:   17:26732190-26732298question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000447110 ENST00000584803 ENST00000581552 ENST00000576273 ENST00000592419 ENST00000590521 ENST00000586777 ENST00000449260 ENST00000552882 ENST00000550464 ENST00000548747 ENST00000548493 ENST00000547137 ENST00000360714 ENST00000539511 ENST00000040738 ENST00000584995 ENST00000584426 ENST00000440501 ENST00000321666 ENST00000262519 ENST00000374170 ENST00000424834 ENST00000382108 ENST00000252242 ENST00000549420 ENST00000557542 ENST00000557263 ENST00000312858 ENST00000261530 ENST00000556663 ENST

Gene Information

This gene encodes a transmembrane proton-coupled folate transporter protein that facilitates the movement of folate and antifolate substrates across cell membranes optimally in acidic pH environments. This protein is also expressed in the brain and choroid plexus where it transports folates into the central nervous system. This protein further functions as a heme transporter in duodenal enterocytes and potentially in other tissues like liver and kidney. Its localization to the apical membrane or cytoplasm of intestinal cells is modulated by dietary iron levels. Mutations in this gene are associated with autosomal recessive hereditary folate malabsorption (HFM) disease. HFM is characterized by folate deficiency due to reduced intestinal folate absorption and subsequent anemia hypoimmunoglobulinemia and recurrent infections. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Jun 2011]

Gene Symbol:   SLC46A1
Gene Name:   solute carrier family 46 (folate transporter), member 1
Aliases:   FLJ39875, HCP1, MGC9564, PCFT
RefSeq:   NC_000017.10 NG_013306.1 NT_010799.15
Ensembl:   ENSG00000076351
Entrez:   113235
Chromosome Mapping:   17q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.400000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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