PrimePCR™ Probe Assay: TMEM161A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TMEM161A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TMEM161A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040651
Assay Design:   exonic
Chromosome Location:   19:19245598-19249236question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000552864 ENST00000277570 ENST00000368020 ENST00000368021 ENST00000435396 ENST00000450333 ENST00000162044 ENST00000587583 ENST00000587096 ENST00000587915 ENST00000371481 ENST00000371483 ENST00000303230 ENST00000394231 ENST00000577810 ENST00000312019 ENST00000305620

Gene Information

Description Not Available

Gene Symbol:   TMEM161A
Gene Name:   transmembrane protein 161A
Aliases:   AROS-29, FLJ20422, FLJ39645
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000064545
Entrez:   54929
Chromosome Mapping:   19p13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.870000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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