PrimePCR™ Probe Assay: TMEM161A, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040651
Assay Design:   exonic
Chromosome Location:   19:19245598-19249236question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000552864 ENST00000277570 ENST00000368020 ENST00000368021 ENST00000435396 ENST00000450333 ENST00000162044 ENST00000587583 ENST00000587096 ENST00000587915 ENST00000371481 ENST00000371483 ENST00000303230 ENST00000394231 ENST00000577810 ENST00000312019 ENST00000305620

Gene Information

Description Not Available

Gene Symbol:   TMEM161A
Gene Name:   transmembrane protein 161A
Aliases:   AROS-29, FLJ20422, FLJ39645
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000064545
Entrez:   54929
UniGene:   Hs.631629
Chromosome Mapping:   19p13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.870000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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