PrimePCR™ Probe Assay: MPPE1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040627
Assay Design:   exonic
Chromosome Location:   18:11897236-11906210question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000399978 ENST00000586844 ENST00000589859 ENST00000588103 ENST00000590501 ENST00000589267 ENST00000588191 ENST00000588186 ENST00000592977 ENST00000344987 ENST00000309976 ENST00000317235 ENST00000588072

Gene Information

Description Not Available

Gene Symbol:   MPPE1
Gene Name:   metallophosphoesterase 1
Aliases:   PGAP5
RefSeq:   NC_000018.9 NT_010859.14
Ensembl:   ENSG00000154889
Entrez:   65258
UniGene:   Hs.712666
Chromosome Mapping:   18p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.670000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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