PrimePCR™ Probe Assay: ST6GAL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ST6GAL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ST6GAL1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040626
Assay Design:   exonic
Chromosome Location:   3:186760473-186760573question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000446170 ENST00000423451 ENST00000416235 ENST00000448044 ENST00000440338 ENST00000448408 ENST00000438590 ENST00000417392 ENST00000430309 ENST00000458216 ENST00000169298

Gene Information

This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein which is normally found in the Golgi but can be proteolytically processed to a soluble form is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6 CD75 and CD76. This gene has been incorrectly referred to as CD75. Three transcript variants encoding two different isoforms have been described. [provided by RefSeq Aug 2009]

Gene Symbol:   ST6GAL1
Gene Name:   ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
Aliases:   MGC48859, SIAT1, ST6GalI, ST6N
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000073849
Entrez:   6480
Chromosome Mapping:   3q27-q28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.220000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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