PrimePCR™ Probe Assay: PDCD6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PDCD6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PDCD6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040552
Assay Design:   exonic
Chromosome Location:   5:314602-314695question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000264933 ENST00000505221 ENST00000507528 ENST00000507473

Gene Information

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor- Fas- and glucocorticoid-induced programmed cell death. In mice deficient for this gene product however apoptosis was not blocked suggesting this gene product is functionally redundant. [provided by RefSeq Jul 2008]

Gene Symbol:   PDCD6
Gene Name:   programmed cell death 6
Aliases:   ALG-2, FLJ46208, MGC111017, MGC119050, MGC9123, PEF1B
RefSeq:   NC_000005.9 NT_006576.16
Ensembl:   ENSG00000249915
Entrez:   10016
Chromosome Mapping:   5p15.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.930000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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