This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: RP9, Human
PrimePCR™ Template for Probe Assay: RP9, Human
The protein encoded by this gene can be bound and phosphorylated by the protooncogene PIM1 product a serine/threonine protein kinase . This protein localizes in nuclear speckles containing the splicing factors and has a role in pre-mRNA splicing. CBF1-interacting protein (CIR) a corepressor of CBF1 can also bind to this protein and effects alternative splicing. Mutations in this gene result in autosomal dominant retinitis pigmentosa-9. This gene has a pseudogene (GeneID: 441212) which is located in tandem array approximately 166 kb distal to this gene. [provided by RefSeq Sep 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.