PrimePCR™ Probe Assay: MBNL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040347
Assay Design:   exonic
Chromosome Location:   3:152132739-152132862question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000355460 ENST00000495875 ENST00000493459 ENST00000324210 ENST00000459747 ENST00000460591 ENST00000498502 ENST00000485910 ENST00000463374 ENST00000465907 ENST00000492948 ENST00000485509 ENST00000464596 ENST00000282486 ENST00000282488 ENST00000324196 ENST00000545754 ENST00000357472

Gene Information

Description Not Available

Gene Symbol:   MBNL1
Gene Name:   muscleblind-like (Drosophila)
Aliases:   DKFZp686P06174, EXP, EXP35, EXP40, EXP42, KIAA0428, MBNL
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000152601
Entrez:   4154
UniGene:   Hs.201858
Chromosome Mapping:   3q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.320000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download