PrimePCR™ Probe Assay: SPAG16, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040345
Assay Design:   exonic
Chromosome Location:   2:214181943-214182046question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000331683 ENST00000432529 ENST00000413312 ENST00000447990 ENST00000272898 ENST00000374309

Gene Information

Cilia and flagella are comprised of a microtubular backbone the axoneme which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells respectively (Zhang et al. 2007 [PubMed 17699735]).[supplied by OMIM Jul 2008]

Gene Symbol:   SPAG16
Gene Name:   sperm associated antigen 16
Aliases:   DKFZp666P1710, FLJ22724, FLJ37717, MGC87036, PF20, WDR29
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000144451
Entrez:   79582
UniGene:   Hs.731948
Chromosome Mapping:   2q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.490000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download