PrimePCR™ Probe Assay: SMUG1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SMUG1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SMUG1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040315
Assay Design:   exonic
Chromosome Location:   12:54577497-54577593question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000508394 ENST00000506595 ENST00000514685 ENST00000505128 ENST00000337581 ENST00000513838 ENST00000243112 ENST00000401977 ENST00000514196 ENST00000504338 ENST00000507904 ENST00000504797 ENST00000506169 ENST00000503306

Gene Information

This gene encodes a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene; the full-length nature is known for some but not all of the variants. [provided by RefSeq Aug 2011]

Gene Symbol:   SMUG1
Gene Name:   single-strand-selective monofunctional uracil-DNA glycosylase 1
Aliases:   FDG, HMUDG, MGC104370, UNG3
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000123415
Entrez:   23583
Chromosome Mapping:   12q13.11-q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.890000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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