PrimePCR™ Probe Assay: AC112502.1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: AC112502.1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AC112502.1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040278
Assay Design:   exonic
Chromosome Location:   3:157820467-157820613question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000342922 ENST00000402799 ENST00000406482 ENST00000349238 ENST00000311027 ENST00000407859 ENST00000395344 ENST00000395336 ENST00000402192 ENST00000554971 ENST00000560001 ENST00000558872 ENST00000381575 ENST00000334060 ENST00000381578 ENST00000556336 ENST00000557771 ENST00000400017 ENST00000206660 ENST00000314191 ENST00000338368 ENST00000375375 ENST00000400661 ENST00000425436 ENST00000490689 ENST00000389589 ENST00000555977 ENST00000441443 ENST00000483851

Gene Information

Description Not Available

Gene Symbol:   AC112502.1
Gene Name:   short stature homeobox protein 2 isoform c
Aliases:   OG12, OG12X, SHOT
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000258518
Entrez:   6474
Chromosome Mapping:   3q25.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.990000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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