PrimePCR™ Probe Assay: PSMB9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040213
Assay Design:   exonic
Chromosome Location:   6:32825835-32826157question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000434083 ENST00000455846 ENST00000427870 ENST00000425855 ENST00000399371 ENST00000415658 ENST00000383114 ENST00000399381 ENST00000548018 ENST00000422904 ENST00000419512 ENST00000453059 ENST00000418861 ENST00000451862 ENST00000417566 ENST00000422729 ENST00000445315 ENST00000548085 ENST00000399607 ENST00000434587 ENST00000383234 ENST00000399619 ENST00000552166 ENST00000420182 ENST00000413480 ENST00000444284 ENST00000448066 ENST00000546747 ENST00000481893 ENST00000541884 ENST00000439630 ENST

Gene Information

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome the immunoproteasome is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family also known as the T1B family that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq Mar 2010]

Gene Symbol:   PSMB9
Gene Name:   proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)
Aliases:   LMP2, MGC70470, PSMB6i, RING12, beta1i
RefSeq:   NC_000006.11 NG_011759.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000240065
Entrez:   5698
UniGene:   Hs.654585
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.600000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download