PrimePCR™ Probe Assay: KRTAP9-2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KRTAP9-2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KRTAP9-2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040212
Assay Design:   exonic
Chromosome Location:   17:39383241-39383418question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000455970 ENST00000377721 ENST00000411528 ENST00000254072 ENST00000334109 ENST00000394008 ENST00000391354 ENST00000571104 ENST00000570861 ENST00000570825 ENST00000573531 ENST00000337299 ENST00000380518 ENST00000262395 ENST00000581396 ENST00000317716 ENST00000478080 ENST00000542731 ENST00000376477 ENST00000309502 ENST00000367236 ENST00000337894 ENST00000574248 ENST00000592900 ENST00000575341 ENST00000571096 ENST00000572513 ENST00000600735

Gene Information

This protein is a member of the keratin-associated protein (KAP) family. The KAP proteins form a matrix of keratin intermediate filaments which contribute to the structure of hair fibers. KAP family members appear to have unique family-specific amino- and carboxyl-terminal regions and are subdivided into three multi-gene families according to amino acid composition: the high sulfur the ultrahigh sulfur and the high tyrosine/glycine KAPs. This protein is a member of the ultrahigh sulfur KAP family and the gene is localized to a cluster of KAPs at 17q12-q21. [provided by RefSeq Jul 2008]

Gene Symbol:   KRTAP9-2
Gene Name:   keratin associated protein 9-2
Aliases:   KAP9.2, KRTAP9.2, MGC130013
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000239886
Entrez:   83899
Chromosome Mapping:   17q12-q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.540000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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