PrimePCR™ Probe Assay: RGL2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RGL2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RGL2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040205
Assay Design:   exonic
Chromosome Location:   6:33263805-33263894question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000288548 ENST00000413136 ENST00000549377 ENST00000383204 ENST00000547127 ENST00000497454 ENST00000444031 ENST00000522948 ENST00000251822 ENST00000338700 ENST00000452084 ENST00000550374 ENST00000416548 ENST00000548778 ENST00000320634 ENST00000290341 ENST00000586115 ENST00000585983 ENST00000585960 ENST00000527429 ENST00000450876 ENST00000391805 ENST00000309958 ENST00000565289

Gene Information

Description Not Available

Gene Symbol:   RGL2
Gene Name:   ral guanine nucleotide dissociation stimulator-like 2
Aliases:   HKE1.5, KE1.5, RAB2L
RefSeq:   NC_000006.11 NG_009876.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000237441
Entrez:   5863
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.120000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download