PrimePCR™ Probe Assay: FAM22E, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040164
Assay Design:   exonic
Chromosome Location:   10:81610435-81610551question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000296861 ENST00000451286 ENST00000381707 ENST00000381697 ENST00000535173 ENST00000342531 ENST00000429828 ENST00000243077 ENST00000602967 ENST00000429984 ENST00000268154 ENST00000399397 ENST00000342528 ENST00000399391 ENST00000422989 ENST00000373624 ENST00000372322 ENST00000400335 ENST00000400338 ENST00000372851 ENST00000541604 ENST00000418929 ENST00000548804 ENST00000337737 ENST00000548198 ENST00000551632 ENST00000262366 ENST00000433375 ENST00000303334 ENST00000394037 ENST00000543856 ENST

Gene Information

Description Not Available

Gene Symbol:   FAM22E
Gene Name:   family with sequence similarity 22, member E
Aliases:   Not Available
RefSeq:   NC_000010.10 NG_012781.1 NT_030059.13
Ensembl:   ENSG00000228570
Entrez:   283008
UniGene:   Not Available
Chromosome Mapping:   10q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 37.670000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download