PrimePCR™ Probe Assay: WDR46, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: WDR46, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WDR46, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040153
Assay Design:   exonic
Chromosome Location:   6:33255186-33255276question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000432933 ENST00000456332 ENST00000383208 ENST00000416777 ENST00000600603 ENST00000601638 ENST00000599732 ENST00000601675 ENST00000221557 ENST00000391842 ENST00000360864 ENST00000353151 ENST00000326043 ENST00000393350 ENST00000398457 ENST00000347800 ENST00000398449 ENST00000361802 ENST00000343687 ENST00000398437 ENST00000340588 ENST00000231706 ENST00000274364 ENST00000514350 ENST00000505766 ENST00000379730 ENST00000374617 ENST00000444176 ENST00000356663 ENST00000333175 ENST00000335154 ENST

Gene Information

Description Not Available

Gene Symbol:   WDR46
Gene Name:   WD repeat domain 46
Aliases:   BING4, C6orf11, FP221
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000227057
Entrez:   9277
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.230000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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