PrimePCR™ Probe Assay: RBMY1J, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RBMY1J, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RBMY1J, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040151
Assay Design:   exonic
Chromosome Location:   Y:24563608-24563757question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000328965 ENST00000335071 ENST00000383020 ENST00000382707 ENST00000303902 ENST00000439108 ENST00000382659 ENST00000382673 ENST00000382658 ENST00000382680 ENST00000382677 ENST00000418956 ENST00000303766 ENST00000454978 ENST00000250831 ENST00000414629 ENST00000445779 ENST00000325722 ENST00000373258 ENST00000278816 ENST00000377681 ENST00000489634 ENST00000435364 ENST00000522172 ENST00000229328 ENST00000046640 ENST00000414524

Gene Information

This gene encodes a protein containing an RNA-binding motif in the N-terminus and four SRGY (serine arginine glycine tyrosine) boxes in the C-terminus. Multiple copies of this gene are found in the AZFb azoospermia factor region of chromosome Y and the encoded protein is thought to be involved in spermatogenesis. Most copies of this locus are pseudogenes although six highly similar copies have full-length ORFs and are considered functional. Four functional copies of this gene are found within inverted repeat IR2; two functional copies of this gene are found in palindrome P3 along with two copies of PTPN13-like Y-linked. [provided by RefSeq Jul 2008]

Gene Symbol:   RBMY1J
Gene Name:   RNA binding motif protein, Y-linked, family 1, member J
Aliases:   Not Available
RefSeq:   NC_000024.9 NG_004755.2 NT_011903.12
Ensembl:   ENSG00000226941
Entrez:   378951
Chromosome Mapping:   Yq11.223

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.860000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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