PrimePCR™ Probe Assay: AC109486.1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: AC109486.1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AC109486.1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040146
Assay Design:   exonic
Chromosome Location:   5:59726565-59726673question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000599809 ENST00000225724 ENST00000553258 ENST00000601706 ENST00000594734 ENST00000355568 ENST00000379992 ENST00000371316 ENST00000594612 ENST00000412930 ENST00000544076 ENST00000395148 ENST00000597084 ENST00000442514 ENST00000598562 ENST00000333139 ENST00000446232 ENST00000411432 ENST00000539061 ENST00000551364 ENST00000548061 ENST00000403396 ENST00000595520 ENST00000557832 ENST00000398726

Gene Information

Description Not Available

Gene Symbol:   AC109486.1
Gene Name:   AC109486.1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000224838
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.960000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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