PrimePCR™ Probe Assay: C1QTNF5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: C1QTNF5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: C1QTNF5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040141
Assay Design:   exonic
Chromosome Location:   11:119209805-119209928question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000445041 ENST00000528368 ENST00000555262 ENST00000556586 ENST00000589235 ENST00000368073 ENST00000326837 ENST00000368074 ENST00000551568 ENST00000338356 ENST00000409223 ENST00000305959 ENST00000339486 ENST00000581585 ENST00000577501 ENST00000536163 ENST00000570646 ENST00000480945 ENST00000575464

Gene Information

This gene encodes a member of the a member of the C1q/tumor necrosis factor superfamily. The encoded protein may be a component of basement membranes and may play a role in cell adhesion. This gene is contained entirely within the 3' UTR of the membrane frizzled-related protein gene on chromosome 11q23 and both genes are expressed from a bicistronic transcript. Mutations in this gene have been associated with late-onset retinal degeneration.[provided by RefSeq May 2010]

Gene Symbol:   C1QTNF5
Gene Name:   C1q and tumor necrosis factor related protein 5
Aliases:   CTRP5, DKFZp586B0621, FLJ30570, LORD
RefSeq:   NC_000011.9 NG_009626.1 NG_012235.1 NT_033899.8
Ensembl:   ENSG00000223953
Entrez:   114902
Chromosome Mapping:   11q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.930000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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