PrimePCR™ Probe Assay: OR2A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040135
Assay Design:   exonic
Chromosome Location:   7:144015750-144015898question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000457772 ENST00000448044 ENST00000169298 ENST00000342868 ENST00000368061 ENST00000315392 ENST00000357727 ENST00000482321 ENST00000428302 ENST00000436030 ENST00000435007 ENST00000437153 ENST00000449633 ENST00000407689 ENST00000404953 ENST00000438769 ENST00000442871 ENST00000411608 ENST00000433769 ENST00000441161 ENST00000434291 ENST00000408906 ENST00000408979 ENST00000408899 ENST00000391496 ENST00000408951 ENST00000409671 ENST00000375809 ENST00000444404 ENST00000457450 ENST00000440063 ENST

Gene Information

Olfactory receptors interact with odorant molecules in the nose to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq Jul 2008]

Gene Symbol:   OR2A1
Gene Name:   olfactory receptor, family 2, subfamily A, member 1
Aliases:   OR2A42
RefSeq:   NC_000007.13 NT_007914.15
Ensembl:   ENSG00000221970
Entrez:   346528
UniGene:   Hs.528398
Chromosome Mapping:   7q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 39.850000
Efficiency 81

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download