PrimePCR™ Probe Assay: C11orf20, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040133
Assay Design:   exonic
Chromosome Location:   11:64068142-64068281question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000320631 ENST00000359393 ENST00000421510 ENST00000568193 ENST00000564962 ENST00000565557 ENST00000565080 ENST00000561589 ENST00000561919 ENST00000567302 ENST00000565268 ENST00000568204 ENST00000569475 ENST00000563699 ENST00000359872 ENST00000539943 ENST00000328404 ENST00000325888 ENST00000346177 ENST00000341529 ENST00000354618 ENST00000469141 ENST00000308860 ENST00000541476 ENST00000300571 ENST00000535671 ENST00000569479 ENST00000569847 ENST00000537135 ENST00000345136 ENST00000357649 ENST

Gene Information

Description Not Available

Gene Symbol:   C11orf20
Gene Name:   chromosome 11 open reading frame 20
Aliases:   DKFZp566E164
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000219435
Entrez:   25858
UniGene:   Hs.97174
Chromosome Mapping:   11q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.580000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download