PrimePCR™ Probe Assay: PPP1R11, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040078
Assay Design:   exonic
Chromosome Location:   6:30037736-30037834question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000433942 ENST00000452679 ENST00000417223 ENST00000436833 ENST00000428563 ENST00000449435 ENST00000441553 ENST00000437937 ENST00000446310 ENST00000414618 ENST00000434591 ENST00000452227 ENST00000455350 ENST00000448378 ENST00000429382 ENST00000418211 ENST00000444101 ENST00000430959 ENST00000376773 ENST00000376772 ENST00000376769 ENST00000376765 ENST00000376763 ENST00000376758 ENST00000430828 ENST00000436591 ENST00000448210 ENST00000444994 ENST00000412077 ENST00000422273 ENST00000400658 ENST

Gene Information

This gene encodes a specific inhibitor of protein phosphatase-1 (PP1) with a differential sensitivity toward the metal-independent and metal-dependent forms of PP1. The gene is located within the major histocompatibility complex class I region on chromosome 6. [provided by RefSeq Jul 2008]

Gene Symbol:   PPP1R11
Gene Name:   protein phosphatase 1, regulatory (inhibitor) subunit 11
Aliases:   HCG-V, HCGV, IPP3, MGC125741, MGC125742, MGC125743, TCTE5, TCTEX5
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204619
Entrez:   6992
UniGene:   Hs.82887
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.970000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download