PrimePCR™ Probe Assay: GNL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GNL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GNL1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040071
Assay Design:   exonic
Chromosome Location:   6:30522832-30523335question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000417834 ENST00000430236 ENST00000310799 ENST00000381569 ENST00000265801 ENST00000437917 ENST00000443235 ENST00000299721 ENST00000383596 ENST00000458592 ENST00000428189 ENST00000435788 ENST00000376621 ENST00000433809 ENST00000441604 ENST00000450423 ENST00000454829 ENST00000416639 ENST00000377647 ENST00000456183 ENST00000356699 ENST00000377509 ENST00000429767 ENST00000377621 ENST00000355832 ENST00000542458 ENST00000332857 ENST00000355045 ENST00000337090 ENST00000347528 ENST00000289734 ENST

Gene Information

The GNL1 gene identified in the human major histocompatibility complex class I region shows a high degree of similarity with its mouse counterpart. The GNL1 gene is located less than 2 kb centromeric to HLA-E in the same transcriptional orientation. GNL1 is telomeric to HLA-B and HLA-C. [provided by RefSeq Jul 2008]

Gene Symbol:   GNL1
Gene Name:   guanine nucleotide binding protein-like 1
Aliases:   DKFZp547E038, DKFZp547E128, FLJ37752, HSR1
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204590
Entrez:   2794
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.270000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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