PrimePCR™ Probe Assay: MUC21, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040066
Assay Design:   exonic
Chromosome Location:   6:30955914-30956004question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000584437 ENST00000457728 ENST00000547599 ENST00000430305 ENST00000547796 ENST00000420532 ENST00000549059 ENST00000354618 ENST00000469141 ENST00000440457 ENST00000551445 ENST00000486149 ENST00000376296 ENST00000451480 ENST00000548519 ENST00000383535 ENST00000551904 ENST00000358136 ENST00000356315 ENST00000011700 ENST00000220244 ENST00000394685 ENST00000356249 ENST00000301011 ENST00000452588 ENST00000567085

Gene Information

Description Not Available

Gene Symbol:   MUC21
Gene Name:   mucin 21, cell surface associated
Aliases:   C6orf205, KMQK697, MGC125609, MUC-21, bCX31G15.2
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000204544
Entrez:   394263
UniGene:   Hs.448277
Chromosome Mapping:   6p21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.030000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download