PrimePCR™ Probe Assay: PRAMEF22, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PRAMEF22, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRAMEF22, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040058
Assay Design:   exonic
Chromosome Location:   1:13037962-13038085question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000436923 ENST00000428494 ENST00000587427 ENST00000414034 ENST00000301671 ENST00000593209 ENST00000447110 ENST00000584803 ENST00000581552 ENST00000378550 ENST00000425673 ENST00000205135 ENST00000409797 ENST00000458508 ENST00000409794 ENST00000527087 ENST00000416121 ENST00000254325 ENST00000358747 ENST00000370332 ENST00000294702 ENST00000427103 ENST00000382499 ENST00000433931 ENST00000382491 ENST00000322229 ENST00000357345 ENST00000316099 ENST00000415691 ENST00000408047 ENST00000377924 ENST

Gene Information

Description Not Available

Gene Symbol:   PRAMEF22
Gene Name:   PRAME family member 22
Aliases:   PRAMEF3L
RefSeq:   NC_000001.10 NT_021937.19
Ensembl:   ENSG00000204508
Entrez:   653606
Chromosome Mapping:   1p36.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.490000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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