PrimePCR™ Probe Assay: PRAMEF5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040057
Assay Design:   exonic
Chromosome Location:   1:13366295-13368174question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000333684 ENST00000352909 ENST00000381175 ENST00000381178 ENST00000342555 ENST00000451024 ENST00000277541 ENST00000344754 ENST00000202578 ENST00000353903 ENST00000294288 ENST00000437584 ENST00000535591 ENST00000235349 ENST00000355096 ENST00000376192 ENST00000376189 ENST00000415464 ENST00000376182 ENST00000414205 ENST00000423177 ENST00000376168 ENST00000376152 ENST00000327795 ENST00000332192 ENST00000330087 ENST00000602960 ENST00000316412 ENST00000221856 ENST00000312358

Gene Information

Description Not Available

Gene Symbol:   PRAMEF5
Gene Name:   PRAME family member 5
Aliases:   Not Available
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000204502
Entrez:   343068
UniGene:   Hs.567864
Chromosome Mapping:   1p36.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.980000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download