PrimePCR™ Probe Assay: NCR3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NCR3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NCR3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040048
Assay Design:   exonic
Chromosome Location:   6:31557762-31557856question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000508394 ENST00000337581 ENST00000401977 ENST00000514196 ENST00000420556 ENST00000432392 ENST00000457547 ENST00000419728 ENST00000406958 ENST00000272972 ENST00000401804 ENST00000391987 ENST00000373320 ENST00000437517 ENST00000436253 ENST00000452296 ENST00000419086 ENST00000430599 ENST00000446756 ENST00000441372 ENST00000453657 ENST00000262415 ENST00000340027 ENST00000376073 ENST00000376072 ENST00000376071 ENST00000383566 ENST00000552349 ENST00000548827 ENST00000550408 ENST00000435674 ENST

Gene Information

The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247) a T-cell receptor. A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq May 2010]

Gene Symbol:   NCR3
Gene Name:   natural cytotoxicity triggering receptor 3
Aliases:   1C7, CD337, LY117, MALS, NKp30
RefSeq:   NC_000006.11 NG_021176.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204475
Entrez:   259197
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.700000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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