PrimePCR™ Probe Assay: ABHD16A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ABHD16A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ABHD16A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040042
Assay Design:   exonic
Chromosome Location:   6:31664725-31668723question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000421022 ENST00000506491 ENST00000508657 ENST00000309755 ENST00000306390 ENST00000432617 ENST00000440799 ENST00000550719 ENST00000549722 ENST00000429735 ENST00000414591 ENST00000546528 ENST00000552042 ENST00000407464 ENST00000407598 ENST00000443632 ENST00000395952 ENST00000440843 ENST00000375842 ENST00000538874 ENST00000457366 ENST00000422411 ENST00000547325 ENST00000549853 ENST00000413853 ENST00000422404 ENST00000549543 ENST00000551038 ENST00000444818 ENST00000431671 ENST00000547161 ENST

Gene Information

A cluster of genes BAT1-BAT5 has been localized in the vicinity of the genes for tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. The protein encoded by this gene is thought to be involved in some aspects of immunity. Alternatively spliced transcript variants have been described. [provided by RefSeq Apr 2010]

Gene Symbol:   ABHD16A
Gene Name:   abhydrolase domain containing 16A
Aliases:   BAT5, D6S82E, NG26, PP199
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204427
Entrez:   7920
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.650000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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