PrimePCR™ Probe Assay: EHMT2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EHMT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EHMT2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040031
Assay Design:   exonic
Chromosome Location:   6:31852169-31852264question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000400006 ENST00000400008 ENST00000383372 ENST00000383373 ENST00000419454 ENST00000550866 ENST00000415323 ENST00000450323 ENST00000440987 ENST00000421926 ENST00000448415 ENST00000551865 ENST00000395728 ENST00000375528 ENST00000375530 ENST00000375537 ENST00000436026 ENST00000425250 ENST00000458593 ENST00000463686 ENST00000436777 ENST00000447162 ENST00000446303 ENST00000427405 ENST00000454705 ENST00000420336 ENST00000424396 ENST00000546877 ENST00000429506 ENST00000450229 ENST00000428442 ENST

Gene Information

A cluster of genes BAT1-BAT5 has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes NG36 and G9a adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described. [provided by RefSeq Jul 2008]

Gene Symbol:   EHMT2
Gene Name:   euchromatic histone-lysine N-methyltransferase 2
Aliases:   BAT8, C6orf30, DKFZp686H08213, FLJ35547, G9A, KMT1C, NG36
RefSeq:   NC_000006.11 NG_023058.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204371
Entrez:   10919
Chromosome Mapping:   6p21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.080000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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