PrimePCR™ Probe Assay: SKIV2L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SKIV2L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SKIV2L, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040029
Assay Design:   exonic
Chromosome Location:   6:31936676-31936791question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000167586 ENST00000582783 ENST00000540488 ENST00000338779 ENST00000576338 ENST00000427522 ENST00000467148 ENST00000448219 ENST00000569795 ENST00000419739 ENST00000287196 ENST00000260376 ENST00000383336 ENST00000396073 ENST00000541296 ENST00000542457 ENST00000333577 ENST00000389561 ENST00000389562 ENST00000332482 ENST00000330386 ENST00000316843 ENST00000375394 ENST00000491994 ENST00000544581 ENST00000504926 ENST00000306315 ENST00000509799 ENST00000513986 ENST00000257974 ENST00000421789 ENST

Gene Information

DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and spliceosome assembly. Based on their distribution patterns some members of this family are believed to be involved in embryogenesis spermatogenesis and cellular growth and division. This gene encodes a DEAD box protein which is a human homologue of yeast SKI2 and may be involved in antiviral activity by blocking translation of poly(A) deficient mRNAs. This gene is located in the class III region of the major histocompatibility complex. [provided by RefSeq Jul 2008]

Gene Symbol:   SKIV2L
Gene Name:   superkiller viralicidic activity 2-like (S. cerevisiae)
Aliases:   170A, DDX13, HLP, SKI2, SKI2W, SKIV2
RefSeq:   NC_000006.11 NG_000013.3 NG_004658.2 NG_005163.2 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204351
Entrez:   6499
Chromosome Mapping:   6p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.210000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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