PrimePCR™ Probe Assay: AGER, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AGER, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AGER, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040022
Assay Design:   exonic
Chromosome Location:   6:32150713-32150898question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000424145 ENST00000426320 ENST00000427822 ENST00000454284 ENST00000418313 ENST00000550885 ENST00000551378 ENST00000448768 ENST00000452945 ENST00000426138 ENST00000436653 ENST00000412794 ENST00000546535 ENST00000552891 ENST00000441180 ENST00000453588 ENST00000436456 ENST00000455471 ENST00000434502 ENST00000552621 ENST00000550562 ENST00000383279 ENST00000383275 ENST00000451115 ENST00000415973 ENST00000453119 ENST00000549168 ENST00000551254 ENST00000412470 ENST00000449037 ENST00000456918 ENST

Gene Information

The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor and besides AGE interacts with other molecules implicated in homeostasis development and inflammation and certain diseases such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms as well as non-protein-coding variants have been described for this gene (PMID:18089847). [provided by RefSeq May 2011]

Gene Symbol:   AGER
Gene Name:   advanced glycosylation end product-specific receptor
Aliases:   MGC22357, RAGE
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204305
Entrez:   177
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.490000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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