PrimePCR™ Probe Assay: PSMB8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040017
Assay Design:   exonic
Chromosome Location:   6:32808671-32808799question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000244533 ENST00000354903 ENST00000556751 ENST00000543004 ENST00000538426 ENST00000394464 ENST00000343796 ENST00000424646 ENST00000446855 ENST00000455660 ENST00000436627 ENST00000446762 ENST00000441960 ENST00000449883 ENST00000546794 ENST00000425784 ENST00000429645 ENST00000416564 ENST00000602795 ENST00000344018 ENST00000457261 ENST00000447073 ENST00000416134 ENST00000383238 ENST00000383236 ENST00000399630 ENST00000394480 ENST00000427919 ENST00000421445 ENST00000452573 ENST00000261233 ENST

Gene Information

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome the immunoproteasome is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family also known as the T1B family that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq Jul 2008]

Gene Symbol:   PSMB8
Gene Name:   proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
Aliases:   D6S216, D6S216E, LMP7, MGC1491, PSMB5i, RING10
RefSeq:   NC_000006.11 NG_009793.1 NG_011759.1 NG_028165.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204264
Entrez:   5696
UniGene:   Hs.180062
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.930000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download