PrimePCR™ Probe Assay: HSD17B8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040011
Assay Design:   exonic
Chromosome Location:   6:33173238-33173440question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000331351 ENST00000427295 ENST00000230236 ENST00000556603 ENST00000555495 ENST00000267615 ENST00000330817 ENST00000347950 ENST00000404286 ENST00000515118 ENST00000358130 ENST00000591090 ENST00000586561 ENST00000357332 ENST00000514182 ENST00000220562 ENST00000374662 ENST00000480944 ENST00000598234 ENST00000423994 ENST00000429770 ENST00000266039 ENST00000360963 ENST00000424201 ENST00000435965 ENST00000395083 ENST00000338432 ENST00000377848 ENST00000377854 ENST00000454191 ENST00000422433 ENST

Gene Information

In mice the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol testosterone and dihydrotestosterone. However the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected but the full-length nature of this variant has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   HSD17B8
Gene Name:   hydroxysteroid (17-beta) dehydrogenase 8
Aliases:   D6S2245E, FABG, FABGL, H2-KE6, HKE6, KE6, RING2, SDR30C1, dJ1033B10.9
RefSeq:   NC_000006.11 NG_023374.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000204228
Entrez:   7923
UniGene:   Hs.415058
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.500000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download