PrimePCR™ Probe Assay: LIME1, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040004
Assay Design:   exonic
Chromosome Location:   20:62369326-62369538question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000429169 ENST00000356738 ENST00000354402 ENST00000357560 ENST00000358580 ENST00000216241 ENST00000444951 ENST00000309546 ENST00000312962 ENST00000361821 ENST00000334530 ENST00000306306 ENST00000400335 ENST00000400338 ENST00000441322 ENST00000453733 ENST00000413657 ENST00000340738 ENST00000554301 ENST00000555445 ENST00000427855 ENST00000381166 ENST00000356577 ENST00000300278 ENST00000381679 ENST00000290239 ENST00000338591 ENST00000413453 ENST00000324941 ENST00000451519 ENST00000576116 ENST

Gene Information

LIME1 is a raft-associated transmembrane adaptor phosphoprotein that is preferentially expressed in hemopoietic cells particularly T cells (Brdickova et al. 2003 [PubMed 14610046]; Hur et al. 2003 [PubMed 14610044]).[supplied by OMIM Mar 2008]

Gene Symbol:   LIME1
Gene Name:   Lck interacting transmembrane adaptor 1
Aliases:   FLJ20406, LIME, LP8067, dJ583P15.4
RefSeq:   NC_000020.10 NT_011333.6
Ensembl:   ENSG00000203896
Entrez:   54923
UniGene:   Hs.233220
Chromosome Mapping:   20q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 34.990000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download