PrimePCR™ Probe Assay: F8A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: F8A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: F8A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039991
Assay Design:   exonic
Chromosome Location:   X:154115738-154115868question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000317568 ENST00000355991 ENST00000416776 ENST00000531633 ENST00000369446 ENST00000369505 ENST00000369445 ENST00000370557 ENST00000318227 ENST00000448843 ENST00000380149 ENST00000597478 ENST00000598873 ENST00000599403

Gene Information

This gene is contained entirely within intron 22 of the factor VIII gene; spans less than 2 kb and is transcribed in the direction opposite of factor VIII. A portion of intron 22 (int22h) containing F8A is repeated twice extragenically closer to the Xq telomere. Although its function is unknown the observation that this gene is conserved in the mouse implies it has some function. Unlike factor VIII this gene is transcribed abundantly in a wide variety of cell types. [provided by RefSeq Jul 2008]

Gene Symbol:   F8A1
Gene Name:   coagulation factor VIII-associated 1
Aliases:   DXS522E, F8A, HAP40
RefSeq:   NC_000023.10 NG_005114.1 NG_011403.1 NT_167198.1
Ensembl:   ENSG00000197932
Entrez:   8263
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.360000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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