PrimePCR™ Probe Assay: KIR2DL4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KIR2DL4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KIR2DL4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039964
Assay Design:   exonic
Chromosome Location:   19:55317577-55317667question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000400846 ENST00000400848 ENST00000400852 ENST00000400853 ENST00000400854 ENST00000344867 ENST00000400851 ENST00000400847 ENST00000391731 ENST00000568225 ENST00000416429 ENST00000525985 ENST00000391728 ENST00000326542 ENST00000358178 ENST00000402254 ENST00000538269 ENST00000541392 ENST00000359085 ENST00000345540 ENST00000357494 ENST00000396293 ENST00000346587 ENST00000396289 ENST00000396284 ENST00000342376 ENST00000434419 ENST00000336077 ENST00000291633 ENST00000571420 ENST00000574061 ENST

Gene Information

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes although several "framework" genes are found in all haplotypes (KIR3DL3 KIR3DP1 KIR3DL4 KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM) while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus KIR proteins are thought to play an important role in regulation of the immune response. This gene is one of the "framework" loci that is present on all haplotypes. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jul 2008]

Gene Symbol:   KIR2DL4
Gene Name:   killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4
Aliases:   CD158D, G9P, KIR103, KIR103AS
RefSeq:   NC_000019.9 NG_005990.1 NG_005994.1 NG_021414.1 NT_011109.16
Ensembl:   ENSG00000189013
Entrez:   3805
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.630000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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