PrimePCR™ Probe Assay: POTEM, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: POTEM, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POTEM, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039949
Assay Design:   exonic
Chromosome Location:   14:19988568-19988716question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000570382 ENST00000571721 ENST00000575659 ENST00000575994 ENST00000575087 ENST00000575842 ENST00000573283 ENST00000331925 ENST00000290378 ENST00000409832 ENST00000551509 ENST00000331789 ENST00000432588 ENST00000443528 ENST00000417101 ENST00000366684 ENST00000366683 ENST00000409914 ENST00000357462 ENST00000451531 ENST00000409602 ENST00000356920 ENST00000358087 ENST00000598366 ENST00000601845 ENST00000593601 ENST00000601143 ENST00000601751 ENST00000598227 ENST00000599059 ENST00000593414

Gene Information

Description Not Available

Gene Symbol:   POTEM
Gene Name:   POTE ankyrin domain family, member M
Aliases:   ACT, P704P, POTE14beta
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000187537
Entrez:   641455
Chromosome Mapping:   14q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 36.080000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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