PrimePCR™ Probe Assay: MAGED4B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAGED4B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAGED4B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039947
Assay Design:   exonic
Chromosome Location:   X:51810408-51810514question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000358495 ENST00000430095 ENST00000536177 ENST00000497164 ENST00000360134 ENST00000485287 ENST00000335504 ENST00000486010 ENST00000431659 ENST00000375626 ENST00000416960 ENST00000261574 ENST00000357602 ENST00000490680 ENST00000469360 ENST00000539640 ENST00000373381 ENST00000323482 ENST00000432802 ENST00000321878 ENST00000026218 ENST00000366513 ENST00000601264 ENST00000595965 ENST00000601229 ENST00000593675 ENST00000600173 ENST00000594654 ENST00000599522

Gene Information

This gene is a member of the MAGED gene family. It is expressed only in brain and ovary and some transcript variants of this gene are specifically expressed in glioma cells. This gene is clustered with other MAGED genes on chromosome Xp11. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq May 2011]

Gene Symbol:   MAGED4B
Gene Name:   melanoma antigen family D, 4B
Aliases:   KIAA1859, MGC3210, MGC88639
RefSeq:   NC_000023.10 NT_011638.13
Ensembl:   ENSG00000187243
Entrez:   81557
Chromosome Mapping:   Xp11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.720000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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