PrimePCR™ Probe Assay: DAZ3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DAZ3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DAZ3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039946
Assay Design:   exonic
Chromosome Location:   Y:26959330-26959427question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000320736 ENST00000234256 ENST00000440411 ENST00000257931 ENST00000548043 ENST00000425394 ENST00000441003 ENST00000359061 ENST00000441555 ENST00000259803 ENST00000375525 ENST00000299206 ENST00000430045 ENST00000370162 ENST00000405239 ENST00000382510 ENST00000426000 ENST00000540248 ENST00000382449 ENST00000382440 ENST00000382433 ENST00000382306 ENST00000449947 ENST00000382294 ENST00000382424 ENST00000400493 ENST00000382431 ENST00000382434 ENST00000382365 ENST00000446723 ENST00000315357 ENST

Gene Information

This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains one copy of the 10.8 kb repeat. [provided by RefSeq Jul 2008]

Gene Symbol:   DAZ3
Gene Name:   deleted in azoospermia 3
Aliases:   MGC126441, pDP1679
RefSeq:   NC_000024.9 NG_004755.2 NG_028145.1 NT_011903.12
Ensembl:   ENSG00000187191
Entrez:   57054
Chromosome Mapping:   Yq11.223

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.080000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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