PrimePCR™ Probe Assay: HLA-DQB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HLA-DQB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HLA-DQB1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039895
Assay Design:   exonic
Chromosome Location:   6:32629896-32632587question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000371174 ENST00000419175 ENST00000419685 ENST00000455520 ENST00000456529 ENST00000418419 ENST00000549642 ENST00000434886 ENST00000430790 ENST00000416192 ENST00000399074 ENST00000422950 ENST00000432856 ENST00000430849 ENST00000417780 ENST00000433801 ENST00000424579 ENST00000547371 ENST00000424530 ENST00000433936 ENST00000399088 ENST00000399065 ENST00000413089 ENST00000457445 ENST00000432486 ENST00000428972 ENST00000442043 ENST00000447979 ENST00000548910 ENST00000393056 ENST00000383121 ENST

Gene Information

HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB) both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes dendritic cells macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide exons 2 and 3 encode the two extracellular domains exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. Alternative splicing results in multiple transcript variants. [provided by RefSeq Sep 2011]

Gene Symbol:   HLA-DQB1
Gene Name:   major histocompatibility complex, class II, DQ beta 1
Aliases:   CELIAC1, HLA-DQB, IDDM1
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000179344
Entrez:   3119
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.180000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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