PrimePCR™ Probe Assay: PRYP4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: PRYP4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRYP4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039872
Assay Design:   exonic
Chromosome Location:   Y:28134475-28134613question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000424718 ENST00000548649 ENST00000584751 ENST00000440248 ENST00000550212 ENST00000303804 ENST00000405140 ENST00000523500 ENST00000303728 ENST00000343584 ENST00000441885 ENST00000432116 ENST00000409168 ENST00000383159 ENST00000549232 ENST00000306589 ENST00000424713 ENST00000550539 ENST00000375244 ENST00000451343 ENST00000375247 ENST00000412618 ENST00000546684 ENST00000539749 ENST00000296387

Gene Information

Description Not Available

Gene Symbol:   PRYP4
Gene Name:   PTPN13-like, Y-linked pseudogene 4
Aliases:   Not Available
RefSeq:   NC_000024.9 NG_004755.2 NT_011903.12
Ensembl:   ENSG00000172283
Entrez:   442866
Chromosome Mapping:   Yq11.223

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.670000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download